Plasmid_Backbone

Part:BBa_K4212066:Design

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-1D-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
    Illegal XbaI site found at 757
    Illegal PstI site found at 1820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
    Illegal PstI site found at 1820
    Illegal NotI site found at 748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 742
    Plasmid lacks a suffix.
    Illegal PstI site found at 1820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 742
    Plasmid lacks a suffix.
    Illegal XbaI site found at 757
    Illegal PstI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1798
    Illegal BsaI.rc site found at 780
    Illegal SapI.rc site found at 964


Design Notes

sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which enables to verify the insertion of target sequences if transformants do not express sfGFP.


Source

Synthetic construct.

References